Construction of a luciferase reporter gene vector containingDAB2IPpromoter
نویسندگان
چکیده
منابع مشابه
Construction of a reporter gene vector for Clostridium beijerinckii using a Clostridium endoglucanase gene.
A beta-1,4-endoglucanase gene (eglA) cloned from C. acetobutylicum P262 was selected for use in the development of a reporter system for C. beijerinckii NCIMB 8052. The reporter plasmid, pER1, was constructed by ligating the promoterless eglA gene into the B subtilis/Clostridium shuttle vector, pFNK1, which can replicate and is stably maintained in C. beijerinckii. The expression of the endoglu...
متن کاملCloning Of Anopheles Gambiae CYP6M2 Gene Promoter And Construction Of Its Luciferase Reporter System
CYP6M2 is a Cytochrome P450 gene involved in the detoxification of multiple classes of public health insecticidesin the malaria mosquito Anopheles gambiae. Some P450 genes are known to be up regulatedby the transcription factorsCnCC/ Keap 1 and (ss)/ (Tgo) in Drosophilamelanogaster. Whether this regulatory mechanism is involved inthe regulation of P450s in Anopheles gambiae is yet to be identif...
متن کاملGenetic Resources Construction and Testing of an Intron-Containing Luciferase Reporter Gene From Renilla reniformis
We describe a new Renilla reniformis luciferase reporter gene, RiLUC, which was designed to allow detection of luciferase activity in studies involving Agrobacteriumbased transient expression studies. The RLUC gene was altered to contain a modified intron from the castor bean catalase gene while maintaining consensus eukaryotic splicing sites recognized by the plant spliceosome. RLUC and RiLUC ...
متن کاملImproved eukaryotic promoter-detection vector carrying two luciferase reporter genes.
BENCHMARKS Understanding gene regulation is of fundamental importance to virtually all biological systems. A gene is regulated through cis-acting regulatory DNA sequences (elements) in conjunction with transacting regulatory proteins. The cis-acting regulatory elements involved in transcription include, for example, the promoter, enhancer, silencer, specific activator binding sites, and specifi...
متن کاملStudying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane biogenesis occurs rapidly and in an inducible manner. We have found that phagocytosis of latex beads is practical for these purposes as cells rapidly synthesize membrane lipids to replenish membrane pools lost as wrapping material during part...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2016
ISSN: 2219-2859
DOI: 10.11569/wcjd.v24.i28.3985